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- W2366608124 abstract "AimFermentation technology of recombinant E.coli expressing human granulocyte colony-stimulating factor (rhG-CSF) with shaking flask was optimized.MethodsThe effects of culture temperature,time of inducing,expression,pH,disslved oxygen,inoculum age,inoculum volume and so on were investigated for rhG-CSF.ResultsThe experimental results show that the optimum conditions are: temperature 30℃,initial pH 7.0～7.2, medium volume 20%, rotating speed 180 r/min, and inoculum age 1.0～1.5(A_(600)). It is available for inducing cultivation when the growth of the bacteria is in the initial logarithm growth phase and expressing 4 hours. Under these culture conditions, the expression and optical density(A_(600)) in shaking flask are 36.5% and 5.85,respectively when the optimized M9 medium is used.ConclusionThe results indicate that the rhG-CSF engineered E.Coli strain has better stability and repeatability which provides reliable data for bullk production of rhG-CSF." @default.
- W2366608124 created "2016-06-24" @default.
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- W2366608124 date "2004-01-01" @default.
- W2366608124 modified "2023-09-26" @default.
- W2366608124 title "Studies on the fermentation technology of recombinant E.coli expressing human granulocyte colony-stimulating factor(hG-CSF) in shaking flask" @default.
- W2366608124 hasPublicationYear "2004" @default.
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