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- W2366620912 abstract "In order to systematic study grasshoppers in Inner Mongolia on a molecular level,the rDNA-ITS2 sequences of 120 individuals from 10 common grassland species of Inner Mongolia were amplified and a 180~197 bp ITS2 sequence was obtained by comparing with known grasshopper's ITS2 in Genbank.The obtained ITS2 sequences were aligned using MEGA 4.0 and the ITS2 sequences of Oedaleus asiaticus populations from 15 different areas were analyzed.The grasshopper's ITS2 sequence showed GC preference.The populations of O.asiaticus from 15 different areas had so little differences that only two individuals varied.The number of variation sites was 11 which were all base substitutions and made up 5.67% of detected sequences.Clustering results of 15 populations showed that 13 populations was firstly clustered with a population from Siziwang Banner,and finally with a population from Bai Banner.These results indicated that ITS2 was a highly conserved sequence and not suitable for research on genetic differentiation below grasshopper specie level.Genetic distances of grasshopper species in the same genus were 0.0000,indicating that ITS2 sequence could not be used for species identification in the same genus.Gomphoceridae was firstly clustered with Arcypteridae,secondly with Oedipodidae and finally with Catantopidae,showing evolutionary relationships among different families." @default.
- W2366620912 created "2016-06-24" @default.
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- W2366620912 date "2013-01-01" @default.
- W2366620912 modified "2023-09-24" @default.
- W2366620912 title "ITS2 Sequence Analysis of Ten Grasshopper Species and Different Geographic Populations of Oedaleus asiaticus in Inner Mongolia Grassland" @default.
- W2366620912 hasPublicationYear "2013" @default.
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