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- W2366625593 abstract "Objective:To immunize mice with eukaryotic plasmid pcDN3.1(+) carrying the DNA sequence of HCV hypervariable region 1 related consensus poly-mimotope and to observe the immune responses. Methods: DNA sequence was synthesized according to the peptides’ sequence of HCV hypervariable region 1 related consensus poly-mimotope and the product was inserted into pcDN3.1(+) to construct recombinant plasmid pcDN3.1-SP. After immunizing the mice separatly with 100 μg and 10 μg (mixed with or without unmethylated CpG) of pcDN3.1-SP i.m., the mice were scraficed, their sera were collected, and then the splenocytes were separated. The antibodies in the sera were assayed with ELISA. After the splenocytes were stimulated with pooled peptides and HCV patients’ sera, CD8 +IFN-γ + T cells were identified with FACS, T cell proliferation was assayed by non-radioactive MTS kit, and cytotoxic T cell assays were performed with the non-radioactive LDH method. Results: After the splenocytes of the immunized mice were restimulated with the synthesized peptides, the T cell proliferated obviously, CD8 +IFN-γ + T cells increased, and strong CTL activity was induced, accompanied with relatively weak humoral immune responses. Conclusion: The synthesized mimotope may contain T and B cell epitopes; unmethylated CpG can improve the responses to DNA immunization." @default.
- W2366625593 created "2016-06-24" @default.
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- W2366625593 date "2005-01-01" @default.
- W2366625593 modified "2023-09-24" @default.
- W2366625593 title "Cellular immune response after immunizing mice with eukaryotic plasmid carrying HCV hypervariable region 1 related gene" @default.
- W2366625593 hasPublicationYear "2005" @default.
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