Matches in SemOpenAlex for { <https://semopenalex.org/work/W2366668597> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2366668597 abstract "Objective:To construct the recombinant adeno-associated virus vector expressing tumor angiogenesis-related gene Dll4 short RNA interference(RNAi) and to use it for the preparation of high-titer virus.Methods:pDC316-Dll4-shRNA plasmid,constructed with Dll4 siRNA,was digested with EcoRI and SalI.A 1 500bp fragment,containing U6 promoter and Dll4 siRNA,was obtained and inserted into the adeno-associated virus vector plasmid pSNAV2.0 digested with EcoRI+SalI.Positive vectors were analyzed through enzyme digestion and DNA sequencing.The recombinant plasmid was transfected into BHK-21 cells using LipofectamineTM2000.G418-resistant cells were obtained and infected with HSV1-rc/△UL2,which have the function of packaging recombinant adeno-associated virus(rAAV).After purification,target vector and virus were collected.Results:The target vector,pSNAV2.0-shRNA-Dll4,was successfully constructed,and the rAAV with a titer of 2.4×1011vg/L(vg:vectorgenome) was obtained.Conclusion:rAAV vector may be used in further investigation of Dll4 function in tumor angiogenesis and gene therapy." @default.
- W2366668597 created "2016-06-24" @default.
- W2366668597 creator A5002790443 @default.
- W2366668597 date "2009-01-01" @default.
- W2366668597 modified "2023-09-27" @default.
- W2366668597 title "Construction of recombined adeno-associated virus vector expressing Dll4 short RNA interference and preparation of high-titer virus" @default.
- W2366668597 hasPublicationYear "2009" @default.
- W2366668597 type Work @default.
- W2366668597 sameAs 2366668597 @default.
- W2366668597 citedByCount "0" @default.
- W2366668597 crossrefType "journal-article" @default.
- W2366668597 hasAuthorship W2366668597A5002790443 @default.
- W2366668597 hasConcept C104317684 @default.
- W2366668597 hasConcept C128319531 @default.
- W2366668597 hasConcept C130123407 @default.
- W2366668597 hasConcept C153911025 @default.
- W2366668597 hasConcept C159047783 @default.
- W2366668597 hasConcept C166703698 @default.
- W2366668597 hasConcept C190232843 @default.
- W2366668597 hasConcept C22744801 @default.
- W2366668597 hasConcept C2522874641 @default.
- W2366668597 hasConcept C2778107364 @default.
- W2366668597 hasConcept C32470452 @default.
- W2366668597 hasConcept C32611913 @default.
- W2366668597 hasConcept C40767141 @default.
- W2366668597 hasConcept C54009773 @default.
- W2366668597 hasConcept C54355233 @default.
- W2366668597 hasConcept C67705224 @default.
- W2366668597 hasConcept C86803240 @default.
- W2366668597 hasConcept C92087593 @default.
- W2366668597 hasConceptScore W2366668597C104317684 @default.
- W2366668597 hasConceptScore W2366668597C128319531 @default.
- W2366668597 hasConceptScore W2366668597C130123407 @default.
- W2366668597 hasConceptScore W2366668597C153911025 @default.
- W2366668597 hasConceptScore W2366668597C159047783 @default.
- W2366668597 hasConceptScore W2366668597C166703698 @default.
- W2366668597 hasConceptScore W2366668597C190232843 @default.
- W2366668597 hasConceptScore W2366668597C22744801 @default.
- W2366668597 hasConceptScore W2366668597C2522874641 @default.
- W2366668597 hasConceptScore W2366668597C2778107364 @default.
- W2366668597 hasConceptScore W2366668597C32470452 @default.
- W2366668597 hasConceptScore W2366668597C32611913 @default.
- W2366668597 hasConceptScore W2366668597C40767141 @default.
- W2366668597 hasConceptScore W2366668597C54009773 @default.
- W2366668597 hasConceptScore W2366668597C54355233 @default.
- W2366668597 hasConceptScore W2366668597C67705224 @default.
- W2366668597 hasConceptScore W2366668597C86803240 @default.
- W2366668597 hasConceptScore W2366668597C92087593 @default.
- W2366668597 hasLocation W23666685971 @default.
- W2366668597 hasOpenAccess W2366668597 @default.
- W2366668597 hasPrimaryLocation W23666685971 @default.
- W2366668597 hasRelatedWork W2245562270 @default.
- W2366668597 hasRelatedWork W2349415525 @default.
- W2366668597 hasRelatedWork W2349645160 @default.
- W2366668597 hasRelatedWork W2351625714 @default.
- W2366668597 hasRelatedWork W2353870252 @default.
- W2366668597 hasRelatedWork W2354011035 @default.
- W2366668597 hasRelatedWork W2356215523 @default.
- W2366668597 hasRelatedWork W2357736139 @default.
- W2366668597 hasRelatedWork W2360269675 @default.
- W2366668597 hasRelatedWork W2361666546 @default.
- W2366668597 hasRelatedWork W2364346232 @default.
- W2366668597 hasRelatedWork W2367734571 @default.
- W2366668597 hasRelatedWork W2368066453 @default.
- W2366668597 hasRelatedWork W2370466810 @default.
- W2366668597 hasRelatedWork W2373177379 @default.
- W2366668597 hasRelatedWork W2376271110 @default.
- W2366668597 hasRelatedWork W2377144116 @default.
- W2366668597 hasRelatedWork W2378821125 @default.
- W2366668597 hasRelatedWork W2379698185 @default.
- W2366668597 hasRelatedWork W2420122227 @default.
- W2366668597 isParatext "false" @default.
- W2366668597 isRetracted "false" @default.
- W2366668597 magId "2366668597" @default.
- W2366668597 workType "article" @default.