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- W2366697789 abstract "Using the recombinant plasmid pMD-α-BGT as template, alpha-bungarotoxin (α-BGT) gene was obtained by PCR amplification. The cloning plasmid pUCm-α-BGT was constructed by ligating α-BGT gene into pUCm-T vector. α-BGT gene fragment was recovered by BamHⅠand Not I restriction digestion of pUCm-α-BGT, and finally ligated into BamHⅠand Not I digested pGEX-4T-1, generating the fusion gene expression vector pGEX-α-BGT. The expression vector was transformed into Escherichia coli BL21 (DE3) and the recombinant bacteria were induced by IPTG. The SDS-PAGE analysis revealed a foreign protein band of 34 kDa, which appeared as the same as predicted molecular weight and accounted for 32.6% of the total bacterial lysate. The results of Western blotting and indirect ELISA showed that the antigenicity of α-BGT fusion protein was similar to that of natural α-BGT protein." @default.
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- W2366697789 date "2008-01-01" @default.
- W2366697789 modified "2023-09-25" @default.
- W2366697789 title "Construction of fusion gene vector of α-BGT and its expression in E.coli BL21(DE3)" @default.
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