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- W2366703582 abstract "Objective To explore the protective effect and mechanisms of mitochondrial ATP sensitive potassium channel opener diazoxide(DZ) preconditioning on hypoxia/reoxygen(H/R) injury of cultured H9C2 myocytes.Methods Cultured H9C2 myocytes were randomly assigned to 5 groups:group Ⅰ(control);group Ⅱ(H/R);group Ⅲ(DZ preconditioning+H/R);group Ⅳ(DZ+MPG preconditioning+H/R);group Ⅴ(DZ+CH preconditioning+H/R).N-2-mercaptopropionylglycine(MPG) is a free radical scavengers,and CH(Chelerythrine) is a selective PKC inhibitor.Except for group Ⅰ and group Ⅱ,the H9C2 myocytes in other groups were subjected to 100 min of hypoxia and 30 min of reoxygen after being pretreated simultaneouly with DZ or both DZ and MPG or both DZ and CH for 10 min and then being washed-out for 20min.The final concentration of DZ,MPG,CH was 200mmol/L,400mmol/L and 2mmol/L respectively.The rate of apoptotic cells,mitochondrial membrane potential and cytochrome C released from mitochondria were respectively measured by Hoechst33258 staining,JC-1 staining and western blotting.Results Preconditioning with DZ significantly decreased the apoptotic rate of H9C2 myocytes(P0.01),suppressed the loss of mitochondrial membrane potential(P0.01) and reduced the release of cytochrome C released from mitochondria(P0.01).Both MPG and CH could eliminate the protective effects of DZ preconditioning.Conclusion Hypoxia/reoxygen injury could induce apoptosis of H9C2 myocytes by decreasing the mitochondrial membrane potential and reducing the release of cytochrome C from mitochondria to cytoplasm.Pre-opening the Mito-KATP channels could induce the protective effects of H9C2 myocytes against hypoxia/reoxygen injury.The mechanisms of DZ preconditioning might be opening Mito-KATP channels,releasing ROS and PKC activation." @default.
- W2366703582 created "2016-06-24" @default.
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- W2366703582 date "2007-01-01" @default.
- W2366703582 modified "2023-09-23" @default.
- W2366703582 title "Protective effect of diazoxide preconditioning on hypoxia/reoxygen injury of cultured H9C2 myocytes" @default.
- W2366703582 hasPublicationYear "2007" @default.
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