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- W2366706784 abstract "Objectives To study the drug resistance of Pseudomonas aeruginosainfecting surgical incisions,to type carbapenem-resistant strains,and to detect metallo-β-lactamase(MBL)genes and integrons with PCR. Methods In total,186 strains of P.aeruginosa were isolated from infected surgical incisions of patients at Kai Luan General Hospital,the Cancer Institute and Hospital of Tianjin Medical University,and He Bei General Hospital from 2010 to 2013.With P.aeruginosa ATCC27853 and Escherichia coli ATCC25922 serving as control strains,the drug resistance of P.aeruginosa was tested with 8antibiotics.ERI-PCR with primers was used to type 62 strains of carbapenem-resistant P.aeruginosa.MBL genes and integrons were detected with IMP-PCR and VIM-PCR. Results Results of drug susceptibility testing indicated the resistance of 186 strains of P.aeruginosato 8antibiotics.P.aeruginosaresistance to gentamicin and piperacillin was 36.02%,its resistance to amikacin and levofloxacin was 34.95%,its resistance to meropenem was 32.8%,its resistance to ciprofloxacin was 32.26%,its resistance to cefepime was 27.42%,and its resistance to ceftazidime was25.27%.Carbapenem-resistant P.aeruginosa was resistant to meropenem at a rate of 98.4%.The 62 strains of carbapenem-resistant P.aeruginosa were multi-drug-resistant at an overall rate of 33.3%.Eleven strains of carbapenem-resistant P.aeruginosa were pan-drug resistant at an overall rate of 6%.Results indicated that carbapenem-resistant P.aeruginosa had a higher rate of drug resistance than drug sensitivity.By year,there were 2strains of pan-drug-resistant P.aeruginosain 2010,2strains in 2011,3strains in 2012,and 4strains in 2013.MBL genes detected in the 186 strains were VIM2 in strains and IMP-25 in 1strain. Conclusion The frequent use of antibiotics over the past few years has led new strains of drug-resistant P.aeruginosa.The MBL gene IMP-25 was detected in 1strain.Studying the epidemiological characteristics and drug resistance of P.aeruginosais crucial to preventing an epidemic." @default.
- W2366706784 created "2016-06-24" @default.
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- W2366706784 date "2014-01-01" @default.
- W2366706784 modified "2023-09-28" @default.
- W2366706784 title "Detection of MBL genes of Pseudomonas aeruginosainfecting surgical incisions and analysis of the drug resistance of P. aeruginosa" @default.
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