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- W2366775373 abstract "Objective To establish loop-mediated isothemal amplification for detecting Streptococcus pneumoniae.Methods Amplification of Streptococcus pneumoniae strains was performed using LAMP technologies.Traditional culture,PCR method,and the LAMP method were used to detect 50 cases of clinical specimens.The detection rates by the 3 methods were compared;the specificity and sensitivity were measured.Results Amplification products were obtained from all the cultures of Streptococcus pneumoniae;no cross reaction with non-Streptococcus was found.The sensitivity of LAMP detection was 102 CFU/mL.Among 50 clinical specimens,LAMP method checked out 9 positive cases of Streptococcus pneumoniae(18.0%),and 4 positive cases were detected out by culture method(8.0%).Conclusion Compared with traditional culture method,LAMP is of strong specificity,high sensitivity,and is convenient,fast,as well as suitable for Streptococcus pneumoniae detection." @default.
- W2366775373 created "2016-06-24" @default.
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- W2366775373 date "2013-01-01" @default.
- W2366775373 modified "2023-09-22" @default.
- W2366775373 title "Detection of Streptococcus pneumoniae by loop-mediated isothermal amplification" @default.
- W2366775373 hasPublicationYear "2013" @default.
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