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- W2366813963 abstract "Neurofilaments are long flexible cytoplasmic protein polymers that are transported rapidly but intermittently along the axonal processes of nerve cells.Current methods for studying this movement involve manual tracking of fluorescently tagged neurofilament polymers in videos acquired by time-lapse fluorescence microscopy.This paper describes an automated tracking method with particle filtering.To increase the efficiency of this approach,it takes advantage of the fact that neurofilament movement is confined within the boundaries of the axon to limit both the orientation and location of the neurofilament in the particle tracking algorithm such that fewer particles than generic particle filtering are generated significantly.It demonstrates the efficacy and efficiency of this method by performing tracking experiments on real time-lapse image sequences of neurofilament movement,and it shows that the method performs well compared to manual tracking by an experienced user.This spatially constrained particle filtering approach should also be applicable to the movement of other axonally transported cargoes." @default.
- W2366813963 created "2016-06-24" @default.
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- W2366813963 date "2014-01-01" @default.
- W2366813963 modified "2023-09-27" @default.
- W2366813963 title "Neurofilaments tracking with particle filtering algorithm" @default.
- W2366813963 hasPublicationYear "2014" @default.
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