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- W2366858721 abstract "Molecular diagnostics methods were used for detecting Bacillus anthraci and identifying virulence genes directly from clinical material and infected beef in a cutaneous anthrax outbreak in Lianyungang City,Jiangsu Province,PR China,2012.Blood and eschar samples were obtained from suspected cutaneous anthrax patients and the beef was collected from the sick cow.The chromosome gene(rpoB)and plasmid genes(pag,capA)of B.anthraci were detected directly from DNA of samples by real-time PCR.The virulence genes(cya,pag,lefand cap)of B.anthraci were identified by conventional PCR and geneticly analyzed by sequencing.The results showed that the identity of DNA from patients' eschar and beef were further confirmed positive to the genes rpoB,pagand capAof B.anthraci.The sequencing results confirmed that the amplified sequences were virulence genes of B.anthraci.The sequences of each virulent gene from patients and beef were identical.The results revealed that this outbreak was caused by virulent strain of B.anthracis transmitted by contacts of the beef of the sick cow." @default.
- W2366858721 created "2016-06-24" @default.
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- W2366858721 date "2013-01-01" @default.
- W2366858721 modified "2023-09-27" @default.
- W2366858721 title "Detection of Bacillusanthracisand identification of virulence genes directly from clinical material and beef in an anthrax outbreak" @default.
- W2366858721 hasPublicationYear "2013" @default.
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