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- W2366896580 abstract "Objective To establish a cell line with resistance to STI571 (K562-R) in vitro by culturing the wild-type K562 cells(K562-W) and explore the potential mechanisms of acquired resistance.Methods K562-W cells were cultured in gradually increased concentrations of STI571. The growth curve was drawn by counting cells. The cytotoxic effects of K562-W and K562-R were analyzed by MTT assay and apoptosis was detected by Hoechest33342 staining. MDR-1 expression examining, sequence analysis and interphasal fluorescence in situ hybridization (I-FISH) were used to study the potential mechanisms of acquired resistance.Results K562-R showed exponential growth in 0.5 μmol/L STI571. 50% inhibiting concentrations(IC50) of K562-R and K562-W(1.64±0.13) μmol/L and (3.32±0.05)μmol/L respectively by MTT assay and there was(2.04±0.16) fold resistant to STI571. The comparison of the cytotoxic effects of K562-W and K562-R treated with different concentration of adriamycin(ADR) or harringtonine (HT) shows no statistic difference. The apoptosis percentage of K562-R treated with different concentration of STI571 significantly decreased as compared with that of K562-W. The MDR-1 expression percentages of K562-R and K562-W with FASC analysis were 2.68% and 1.39% respectively(P0.001). No point mutant in the Bcr/Abl ATP-binding site was detected and the copies of Bcr/Abl fusion gene were found to increase in K562-R by I-FISH analysis(P0.001).Conclusion In vitro, we establish a resistant cell line. The resistant mechanism of K562-R involves amplification of Bcr/Abl fusion gene." @default.
- W2366896580 created "2016-06-24" @default.
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- W2366896580 date "2008-01-01" @default.
- W2366896580 modified "2023-09-26" @default.
- W2366896580 title "Study on the resistant mechanisms of K562 cells induced by STI571" @default.
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