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- W2366998416 abstract "Objective To investigate the effects of Rho-kinase on ischemia/reperfusion-induced cardiac apoptosis and the effects of the Rho-kinase inhibitor,fasudil on cardiac apoptosis in cultured neonatal rat cardiomyocytes.Methods Neonatal rat ventricular myocytes were prepared from hearts of 1-2 day old Sprague Dawley rat pups,and then subjected to ischmemia and reperfusion injury in vitro.Cultured neonatal cardiomyocytes were visualized with immunofluorescence staining.Cultured cardiomyocytes were randomly divided into three groups: the control group,the I/R group,and the I/R+fasudil group(treated with three concentrations of 10 μmol/L,30 μmol/L and 50 μmol/L).The extent of phosphorylation of myosin phosphatase target subunit 1(MYPT1) was quantified by Western blot analysis,and the activity of the Rho-kinase.Cardiac myocyte apoptosis ratio was determined by flow cytometry with Annexin-V and propidium iodide(PI)staining.Results Cardiomyocyte ischemia reperfusion resulted in a 5.7-fold increase in the amount of phosphorylated MYPT1 compared with the control group(P0.01).In contrast,treatment with fasudil at 10 μmol/L,30 μmol/L and 50 μmol/L attenuated the amount of phosphorylated MYPT1 by 24.6%,40.1% and 60.1%,respectively(P0.05).The cardiac myocyte apoptosis ratio were significantly decreased when myocytes was subjected to 3 h and 6 h reperfusion in the fasudil group in a dose-dependent fashion compared with the I/R group.Conclusions Rho-kinase induces cardiomyocyte apoptosis,however,the Rho-kinase inhibitor,fasudil can decrease cardiomyocyte apoptosis andprotect cardiac cells." @default.
- W2366998416 created "2016-06-24" @default.
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- W2366998416 date "2008-01-01" @default.
- W2366998416 modified "2023-09-23" @default.
- W2366998416 title "Effects of Rho-kinase on cardiac apoptosis induced by ischemia/reperfusion injury in neonatal rat cardiomyocytes" @default.
- W2366998416 hasPublicationYear "2008" @default.
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