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- W2367055932 abstract "Objective To extract, isolate, purify and identify the active components of Bacille Calmette-Guerin (BCG), and to analyze the killing effect of mouse spleen lymphocytes induced by active BCG extracts to human bladder cancer cell line T24 in vitro. Methods Live BCG was divided by the enzyme and ultrasonic method to extract the active protein. The components of BCG extracts was isolated, and analyzed for the molecular weight by Sephadex gel filtration chromatography, then further studied to identify the purity and the molecular weight by the SDS-PAGE gelatin electrophoresis. The killing activity of mouse spleen lymphocytes induced by the active component BCGE2 to the human bladder cancer cell line T24 was analyzed by MTT method. Results The mouse spleen lymphocytes induced by BCGE2 could kill the bladder cancer cell line T24 in vitro. When the effect cells to the target cells was 20:1, the inhibit rate was 42.31%, which was significantly higher than 13.32% in the control group (P0.01). Conclusion The mouse spleen lymphocytes induced by BCGE2 have obvious killing effect on the bladder cancer cell T24 in dose-dependent manner." @default.
- W2367055932 created "2016-06-24" @default.
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- W2367055932 date "2012-01-01" @default.
- W2367055932 modified "2023-09-25" @default.
- W2367055932 title "Killing effect of mouse spleen lymphocytes induced by active BCG extracts to human bladder cancer cell in vitro" @default.
- W2367055932 hasPublicationYear "2012" @default.
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