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- W2367132502 abstract "A large human synthetic phage displayed human library(Tomlinson J) was employed to generate single-chain antibodies(scFvs) against Bacillus thuringiensis(Bt) Cry1B toxin by affinity panning.Specific anti-Cry1B toxin antibodies were isolated from amplified naive phage-displayed human single-fold scFv Tomlinson J library after four rounds of adsorption-elution-amplification by using Cry1B toxin protein as immobilized antigen.Monoclonal phage enzyme-linked immunosorbent assay(ELISA) was used for the positive clones identification by picking single colonies randomly from the final round of panning.The positive clones were confirmed by PCR,DNA electrophoresis and sequencing.Totally 8 positive clones with distinct nucleotide sequences and intact scFv gene were confirmed to be specific for the Cry1B recognition.The positive clone,namely 1E2,which showed better binding ability than others,was employed to develop an indirect competitive ELISA for the detecting of Cry1B.The results indicated that the IC50 reached 1.075 μg/ml,and the minimum detection limit was 0.013 4 μg/ml for the determination of Cry1B.The linear range of detection was approximately 0.5-4.0 μg/ml." @default.
- W2367132502 created "2016-06-24" @default.
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- W2367132502 date "2012-01-01" @default.
- W2367132502 modified "2023-09-24" @default.
- W2367132502 title "Screening and identification of single-chain antibodies(scFvs) against Bacillus thuringiensis Cry1B toxin" @default.
- W2367132502 hasPublicationYear "2012" @default.
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