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- W2367145540 abstract "AIM: To identify the feasibility of using polymerase chain reaction(PCR) for detection of Mycobacterium tuberculosis(MTB) DNA in biopsy tissues and surgical specimens in the form of paraffin blocks from the patients with Crohn disease(CD) or intestinal tuberculosis(ITB).METHODS: DNA was amplified by PCR using the primers selected from IS6110 in MTB chromosome in ITB and CD specimens.The PCR products were confirmed by polyacrylamide gel electrophoresis and PCR-direct sequencing.The relationship between PCR results and pathological features was also analyzed.RESULTS: MTB DNA was positive in 32% and 0% cases of the pathologically or clinically confirmed 25 ITB and 25 CD patients,respectively.The sensitivity of MTB DNA PCR for differential diagnosis of ITB and CD was 32%,significantly higher than that of acid fast staining(8%) and caseation necrosis(12%).Specificity,positive and negative predictive values were 100%,100% and 59.5%,respectively.The positive rate of MTB DNA PCR was higher in the specimens with granulation or multinucleated giant cells in our study,although no statistical difference was observed.The sequencing results coincided with the PCR results.CONCLUSION: PCR can be used to determine MTB in ITB specimens.It is a rapid and convenient assay for the differential diagnosis of ITB and CD." @default.
- W2367145540 created "2016-06-24" @default.
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- W2367145540 date "2013-01-01" @default.
- W2367145540 modified "2023-09-23" @default.
- W2367145540 title "Feasibility of polymerase chain reaction for detecting Mycobacterium tuberculosis DNA in differential diagnosis of intestinal tuberculosis and Crohn disease" @default.
- W2367145540 hasPublicationYear "2013" @default.
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