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- W2367146812 abstract "Objective To establish human HBV/HBx-knockin-p53 rat embryonic stem(ES) cell line,so as to lay a foundation for establishment of animal models.Methods First we constructed the targeting vector pKO-gHBV or pKO-X with HBV whole genome or X and p53 homologue arms by PCR and connection with common gene targeted vector pKO;then after linearized with SalⅠ the vector was transferred into rat ES cells through electroporation.And 2i ES culture medium containing puromycin was used for a three-cycle selection of puromycin resistant clones.Then PCR was used to screen the obtained positive clones;mycoplasma examination and karyotypic analysis were used to confirm targeted ES cell clones.Results We successfully obtained two targeted vectors: pKO-gHBV and pKO-X;then after three-cycle drug-selection we obtained 2 pKO-X clones and 1 pKO-gHBV clone,which were confirmed by PCR,mycoplasma examination and karyotypic analysis.Conclusion We have successfully established human HBV/HBx-knockin-p53 rat ES cells,paving a way for future establishment of HBV/HBx-knockin-p53 rat model." @default.
- W2367146812 created "2016-06-24" @default.
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- W2367146812 date "2012-01-01" @default.
- W2367146812 modified "2023-09-23" @default.
- W2367146812 title "Establishment of human HBV/HBx-Knockin-p53 rat embryonic stem cell line" @default.
- W2367146812 hasPublicationYear "2012" @default.
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