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- W2367179291 abstract "Objective To investigate recombinant adeno-associated virus serotype 2 (rAAV2)-mediated β-galactosidase and human erythropoietin(hEPO) gene transfer to human embryonic cell 293 (HEK293) and human submandibular gland (HSG) cell in vitro. Methods A recombinant adeno-associated virus serotype 2 encoding β-galactosidase ( rAAV2LacZ ) or human erythropoietin ( rAAV2hEPO ) was constructed. The LacZ and hEPO gene were transfered into HEK293 and HSG cells. The expression of β-galactosidad was detected by histochemical staining and the hEPO detected by ELISA methods. Results Expression of β-galactosidase was found in 68.2% of HEK293 cells and 66.3% of HSG cells 24 hours after rAAV2LacZ infection. Human EPO level was virus dose dependent after in vitro infection of HEK293 and HSG cells. Conclusion Recombinant AAV2 can transfer a foreign gene into salivary gland cells in vitro." @default.
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- W2367179291 date "2006-01-01" @default.
- W2367179291 modified "2023-09-27" @default.
- W2367179291 title "Recombinant adeno-associated virus serotype 2-mediated gene transfer to human embryonic cell 293 and human submandibular gland cells" @default.
- W2367179291 hasPublicationYear "2006" @default.
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