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- W2367240905 abstract "The genetic variations,relationships and classification of 4 “KUDINGCHA” species and a few of intermediate-typed materials in Ilex L.of Aquifoliaceae was studied based on RAPD analysis.22 germplasm materials of 4 KUDINGCHA species,i.e.Ilex kudingcha C.J.Tseng,I.latifolia Thunb.,I.pentagona S.K.Chen,Y.X.Feng et C.F.Liang,I.cornuta Lindl.and an intermediate type (it lies between Ilex kudingcha and I.pentagona,in morphology) were analyzed for PCR amplification.27 10-bp random primers with optimal polymorphism were selected from 40 primers tested,and the selected primers were used for the PCR amplification on the 22 germplasm materials mentioned above.445 bands were obtained,of which 432 bands were polymorphic bands,amounting to 97.08% of the total bands amplified.The genetic distances between any two germplasm materials were calculated based on the bands,and the dendrogram was constructed using Nei's genetic similarity values and UPGMA program.The results showed that the 22 germplasm materials can be divided into 4 taxa and 5 groups.Taxon Ⅰ consists of Group A and Group B of pentagona.Group A is a new varietas,var.mashanensis;and Group B is the original varietas,var.pentagona.Taxon Ⅱ,Ⅲ and Ⅳ consist of one Group (one species),respectively.The differences and relationships of species,as well as the differences of origin regions of different germplasm materials of the same species,are clearly reflected in the cluster analysis dendrogram.The results of RAPD analysis can be considered as an important basis for deciding the origin region,genetic variations,and relationship of the various germplasm materials of “KUDINGCHA” in Ilex L.,and for the classification on the species-level or infraspecific level." @default.
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- W2367240905 date "2004-01-01" @default.
- W2367240905 modified "2023-09-25" @default.
- W2367240905 title "A Study on Genetic Variations,Relationships and Classificationof Several “KUDINGCHA” Species in Ilex(Aquifoliaceae) Based on RAPD Analysis" @default.
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