Matches in SemOpenAlex for { <https://semopenalex.org/work/W2367283516> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2367283516 abstract "Objective To construct LC3 expression vector with CFP(cherry fluorescent protein) tag as well as verify the expression. Methods The LC3 sequence was amplified by PCR with pCMV-GFP-LC3 plasmid as template respec-tively. Purified PCR product LC3 was linked with revised pm-cherry-c1 vector digested by restriction enzymes ApaI and BamHI via T4 DNA ligase. The recombined plasmids named PM-CFP-LC3 and PM-CFP-LC3 were identified by sequencing, and then transfected into 293 cells by fugene6 oligfectamine reagent. The distribution of CFP in cells and the expression of LC3 was detected by fluorescence microscopy. Results The eukaryotic expression vector of LC3 with CFP tag was successfully constructed. The cherry fluorescence and the phenomenon of cell autophagy were detected by fluorescence microscopy. Conclusion Recombinant plasmid LC3 with CFP tag has been successfully constructed and expressed, which could be the basis of experiment in cell autophagy." @default.
- W2367283516 created "2016-06-24" @default.
- W2367283516 creator A5065229043 @default.
- W2367283516 date "2012-01-01" @default.
- W2367283516 modified "2023-09-27" @default.
- W2367283516 title "Expression vector construction of LC3 autophagy with CFP tag" @default.
- W2367283516 hasPublicationYear "2012" @default.
- W2367283516 type Work @default.
- W2367283516 sameAs 2367283516 @default.
- W2367283516 citedByCount "0" @default.
- W2367283516 crossrefType "journal-article" @default.
- W2367283516 hasAuthorship W2367283516A5065229043 @default.
- W2367283516 hasConcept C104317684 @default.
- W2367283516 hasConcept C121332964 @default.
- W2367283516 hasConcept C128319531 @default.
- W2367283516 hasConcept C142613039 @default.
- W2367283516 hasConcept C153911025 @default.
- W2367283516 hasConcept C169274487 @default.
- W2367283516 hasConcept C190283241 @default.
- W2367283516 hasConcept C203522944 @default.
- W2367283516 hasConcept C22744801 @default.
- W2367283516 hasConcept C33161422 @default.
- W2367283516 hasConcept C40767141 @default.
- W2367283516 hasConcept C42174275 @default.
- W2367283516 hasConcept C54009773 @default.
- W2367283516 hasConcept C54355233 @default.
- W2367283516 hasConcept C552990157 @default.
- W2367283516 hasConcept C62520636 @default.
- W2367283516 hasConcept C86803240 @default.
- W2367283516 hasConcept C91881484 @default.
- W2367283516 hasConcept C92087593 @default.
- W2367283516 hasConceptScore W2367283516C104317684 @default.
- W2367283516 hasConceptScore W2367283516C121332964 @default.
- W2367283516 hasConceptScore W2367283516C128319531 @default.
- W2367283516 hasConceptScore W2367283516C142613039 @default.
- W2367283516 hasConceptScore W2367283516C153911025 @default.
- W2367283516 hasConceptScore W2367283516C169274487 @default.
- W2367283516 hasConceptScore W2367283516C190283241 @default.
- W2367283516 hasConceptScore W2367283516C203522944 @default.
- W2367283516 hasConceptScore W2367283516C22744801 @default.
- W2367283516 hasConceptScore W2367283516C33161422 @default.
- W2367283516 hasConceptScore W2367283516C40767141 @default.
- W2367283516 hasConceptScore W2367283516C42174275 @default.
- W2367283516 hasConceptScore W2367283516C54009773 @default.
- W2367283516 hasConceptScore W2367283516C54355233 @default.
- W2367283516 hasConceptScore W2367283516C552990157 @default.
- W2367283516 hasConceptScore W2367283516C62520636 @default.
- W2367283516 hasConceptScore W2367283516C86803240 @default.
- W2367283516 hasConceptScore W2367283516C91881484 @default.
- W2367283516 hasConceptScore W2367283516C92087593 @default.
- W2367283516 hasLocation W23672835161 @default.
- W2367283516 hasOpenAccess W2367283516 @default.
- W2367283516 hasPrimaryLocation W23672835161 @default.
- W2367283516 hasRelatedWork W2035085696 @default.
- W2367283516 hasRelatedWork W2284066099 @default.
- W2367283516 hasRelatedWork W2350187512 @default.
- W2367283516 hasRelatedWork W2350732507 @default.
- W2367283516 hasRelatedWork W2359362944 @default.
- W2367283516 hasRelatedWork W2360486978 @default.
- W2367283516 hasRelatedWork W2367465323 @default.
- W2367283516 hasRelatedWork W2371820180 @default.
- W2367283516 hasRelatedWork W2371864295 @default.
- W2367283516 hasRelatedWork W2375278168 @default.
- W2367283516 hasRelatedWork W2376030988 @default.
- W2367283516 hasRelatedWork W2377611564 @default.
- W2367283516 hasRelatedWork W2380691007 @default.
- W2367283516 hasRelatedWork W2383069129 @default.
- W2367283516 hasRelatedWork W2386435243 @default.
- W2367283516 hasRelatedWork W2390096018 @default.
- W2367283516 hasRelatedWork W2392354016 @default.
- W2367283516 hasRelatedWork W2392590349 @default.
- W2367283516 hasRelatedWork W2393400224 @default.
- W2367283516 hasRelatedWork W33738171 @default.
- W2367283516 isParatext "false" @default.
- W2367283516 isRetracted "false" @default.
- W2367283516 magId "2367283516" @default.
- W2367283516 workType "article" @default.