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- W2367285186 abstract "The cell membrane injury and mechanism of 20 nm,60 nm SiO2 nanoparticles on RAW264.7 cells were evaluated in vitro.RAW264.7 cells were treated by 20 nm,60 nm SiO2 nanoparticles and micro SiO2 particles for 24 h.The viability of RAW264.7 cell was measured by MTT assay.The morphology and ultrastructural change were observed by light,fluorescence microscope and transmission electron microscopy.Membrane fluidity of the cells were measured through fluorescence recovery after photobleaching( FRAP) technique with( 2-( 6-( 7-nitrobenz-2-oxa-1,3-diazol-4-yl ) amino ) hexanoyl-1-hexadecanoyl-sn-glycero-3-phosphocholine)(NBD-C6-HPC) as the fluorescent probe.Lactate dehydrogenase( LDH) in culture medium of RAW264.7 cells were assayed by pyruvic acid method.The content of reactive oxygen species( ROS) in RAW264.7 cells were measured by fluorescence probe 2′,7′-chlorodihydrofluorescein diacetate(DCFH-DA).The IC50 of 20-nm,60 nm SiO2 nanoparticles and micro SiO2 were 2.07,6.82 and 14.71 mg /mL,respectively.The cell number decreased,ballooning degeneration and mitochondria swelling after cells treated by three kinks of particles.Si elements were detected in lysosomes and intercellular spaces by energyspectrometer.The fluorescence recovery rate were lower than control( P 0.05 ) after RAW264.7 cells exposured to 20 nm、60 nm SiO2 nanoparticles at 31.25,125,500 μg /mL and micro SiO2 at 500 μg /mL for 24 h.The diffusion coefficients of 20 nm,60 nm SiO2 nanoparticles at 125.00,500.00 μg /mL respectively and micro SiO2 at 500.00 μg /mL were lower than that of the control( P 0.05).The production of LDH was increased compared with the control(P 0.05).The decrease of fluorescence recovery rate and the increase of LDH in 20 nm SiO2 nanoparticles group were much more obvious than 60 nm SiO2 nanoparticles and micro SiO2 groups.The level of ROS in RAW264.7 cells were increased obviously after treated by 20 nm,60 nm SiO2 nanoparticles and micro SiO2 particles(P 0.05).Oxidative stress may be the mechanism of RAW264.7cell growth inhibition,morphological abnormality,ultrastructural change and membrane damage induced by 20 nm and 60 nm SiO2 nanoparticles.20 nm SiO2 nanoparticles were found to be more cytotoxicity than the 60 nm SiO2 nanoparticles.Different cytotoxicity effect induced by SiO2 nanoparticles was presented due to different size.The size effect is a noteworthy issue for the further toxicity research and standard formulation of SiO2 nanoparticles." @default.
- W2367285186 created "2016-06-24" @default.
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- W2367285186 date "2010-01-01" @default.
- W2367285186 modified "2023-09-25" @default.
- W2367285186 title "Cell Membrane Injury Induced by Different Sizes of SiO_2 Nanoparticles in vitro" @default.
- W2367285186 hasPublicationYear "2010" @default.
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