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- W2367318891 abstract "Objective:To establish a multiple PCR system,being able to detect four kinds of food-borne pathogens quickly and accurately.It consists of Staphylococcus aureus,E.coli O157,Proteus vulgaris and Vibrio parahaemolyticus.Methods:Four primer were designed according to the target DNA sequences from above four kinds of pathogens.A multiplex PCR system was developed which can successful amplify four bacteria at the same time.Results:The results showed this multiplex PCR could effectively amplify the corresponding gene fragments.The sensitivity of the pathogens could reach 103CFU/ml.Conclusion:The established multiplex PCR method exhibit more special,rapid,practical in detecting the above four pathogenic bacteria in foods." @default.
- W2367318891 created "2016-06-24" @default.
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- W2367318891 date "2012-01-01" @default.
- W2367318891 modified "2023-09-25" @default.
- W2367318891 title "Development and application of the multiplex PCR method for the detection of Staphylococcus aureus,E.coli O157,Proteus vulgaris and Vibrio parahaemolyticus" @default.
- W2367318891 hasPublicationYear "2012" @default.
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