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- W2367320509 abstract "Objective To study the effect of S100A4 gene cell cycle and cell invasion of laryngeal cancer Hep2 cells by silencing S100A4 gene using RNA interference technique.Methods Hep2 cells were transfected with S100A4 siRNA and the negative control siRNA by lipofectamine 2000.Real-time PCR and Western blot analysis were performed to determine the expression of S100A4 mRNA and protein respectively.Cell cycle distribution and cell invasion of Hep2 cells were detected by flow cytometry assay and transwell,respectively.Results The expression levels of S100A4 mRNA and protein in laryngeal cancer Hep2 cells were downregulated obviously by S100A4 siRNA transfection,the cell cycle was arrested in G1 phase and the cell invasion of Hep2 was inhibited when S100A4 was silenced.Conclusion S100A4 siRNA in laryngeal cancer Hep2 cells can effectively silence S100A4 gene expression,influence cell cycle and inhibit cell invasion of Hep2 cells." @default.
- W2367320509 created "2016-06-24" @default.
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- W2367320509 date "2014-01-01" @default.
- W2367320509 modified "2023-09-28" @default.
- W2367320509 title "Effect of RNA interference of S100A4 gene on cell cycle and invasion in hep2 cells" @default.
- W2367320509 hasPublicationYear "2014" @default.
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