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- W2367331386 abstract "Objective:To study the influence of the traditional Chinese medicine Prunella vulgaris on Fas Ligand mRNA expression of human colon cancer cell line SW480 and invasive ability. Methods: The inhibition concentration 50% (IC50) of Prunella vulgaris was detected with MTT assay.Different concentrations(0.5IC50、IC50) of Prunella vulgaris was used in the cultured human colon cancer cell line SW480.The changes of Fas ligand mRNA were detected by the reverse transcription polymerase chain reaction method(RT-PCR)and invasive ability of SW480 cells was measured by Transwell-ECM. Results: RT-PCR results revealed that the higher concentration Prunella vulgaris upregulated Fas Ligand mRNA expression on SW480 cells(P0.05);Transwell-ECM showed that the higher concentration Prunella vulgaris increased apoptosis rate of lymphocyte induced by SW480 cells(P0.05).The comparisons of different concentrations qroups of Prunella vulgaris had statistical significance(P0.05).Experimental set up three groups,each repeated three samples.Group A control group of 1640,B group Prunella 0.5 IC50 group,C group Prunella IC50 group. Conclusion: Prunella vulgaris can upregulate functional Fas ligand mRNA expression of human colon cancer cell line SW480.At the same time,the upregulative action can raise invasive ability of cancer cells." @default.
- W2367331386 created "2016-06-24" @default.
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- W2367331386 date "2009-01-01" @default.
- W2367331386 modified "2023-09-23" @default.
- W2367331386 title "Influence of the traditional Chinese medicine Prunella vulgaris on Fas ligand gene expression and invasive ability of human colon cancer cells" @default.
- W2367331386 hasPublicationYear "2009" @default.
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