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- W2367337555 abstract "Virus protein VP2 is a minor structural protein of rabbit hemorrhagic disease virus(RHDV).The objective of the present study was to study the expression and intracellular localization of VP2 in eukaryotic cells by fusion with enhanced green fluorescent protein(EGFP).The GST-VP2 fusion protein expressed in E.coli was purified and inoculated into mice.The polyclonal murine antibody was prepared and the specificity to VP2 was determined.To explore the intracellular localization of VP2,the recombinant plasmid(pEGFP-VP2)fused with EGFP was constructed and transfected into BHK-21 cells.The expression of EGFP-VP2 protein was detected by Western blot assay.The intracellular localization of VP2 was observed under fluorescence microscope.Besides,the localization of VP2 was further examined by staining cells with DAPI.The recombinant plasmids were constructed and the fusion proteins were expressed in BHK-21 cells.The VP2 and EGFP were co-localized in BHK-21cells and VP2 was primarily localized in cytoplasm as determined by using indirect immunofluorescence assay(IFA) and DAPI staining.The construct of fusion expression vector of pEGFP-VP2 and expression in eukaryotic cells have laid the foundation for studying the biological function of VP2." @default.
- W2367337555 created "2016-06-24" @default.
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- W2367337555 date "2009-01-01" @default.
- W2367337555 modified "2023-09-24" @default.
- W2367337555 title "INTRACELLULAR LOCALIZATION OF THE MINOR STRUCTURAL PROTEIN(VP2) OF RABBIT HEMORRHAGIC DISEASE VIRUS(RHDV)" @default.
- W2367337555 hasPublicationYear "2009" @default.
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