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- W2367343670 abstract "Objective To obserbve the effects of S-nitroso-N-acetylpenicillamine(SNAP) on growth hormone(GH) secretion and cell proliferation in rat GH3 cells and explore the possible mechanism.Methods GH3 cells were divided into A,B,C,D group.GH3 cells in group A were cultured normally.GH3 cells in group B,C,D were cultured with ghrelin,SNAP,ghrelin+SNAP.After 0,1,2,3,4,5,6 d,MTT was used to detected the absorbance value.After cultured for 2 h,ELISA was used to detected the GH in GH3 cells culture solution;Western blot was used to detected the phospho-excelluar signal-regulated kinase(p-ERK) in GH3 cells.Results The absorbance value of GH3 cells cultured for 2,3,4,5,6 d in group A were 0.381±0.006,1.664±0.005,3.411±0.005,5.221±0.005,10.055±0.005;1.062±0.005,3.115±0.005,4.512±0.005,6.656±0.005,11.060±0.005 in group B;0.161±0.006,0.413±0.005,0.931±0.005,1.861±0.005,5.612±0.005 in group C;0.219±0.004,0.865±0.005,1.712±0.005,3.059±0.005,6.561±0.005 in group D.There were significant difference between the absorbance value of GH3 cells in group B and A,C and A,D and B(all P0.01).The GH level in group A,B,C,D cultured for 2 h were 0.080±0.002,0.165±0.011,0.041±0.003,0.106±0.005;and the p-ERK were 0.301 8±0.004 5,0.682 5±0.003 8,0.192 3±0.008 6,0.298 5±0.006 7.There were significant difference between the GH level and p-ERK in GH3 cells in group B and A,C and A,D and B(all P0.01).Conclusion SNAP reduces both basal and induced by ghrelin GH secretion and cell proliferation in rat GH3 cells via blocking ERK signaling pathway." @default.
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- W2367343670 date "2012-01-01" @default.
- W2367343670 modified "2023-09-22" @default.
- W2367343670 title "Effect and mechanism of SNAP on growth hormone secretion and cell proliferation in rat GH3 cells" @default.
- W2367343670 hasPublicationYear "2012" @default.
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