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- W2367367032 abstract "AIM:To establish a HPLC-MS method for the determination of cyclosporin A in ocular tissues of aqueous humor, vitreous humor, cornea, sclera, iris and lens. METHOD:Ocular tissue samples containing cyclosporin D as internal standard was extracted with ethyl ether. The high-performance liquid chromatography separation was accomplished on RP-C_ 18 column(2.1 mm×150 mm, 5 μm) with the mobile phase of methanol-water(90∶10) at flow rate of 0.25 ml/min. The quantification was carried out with the selected ion recording mode by monitoring the molecular ion CsA m/z 1225 [M+Na] + ,and CsD m/z 1239 [M+Na] + .RESULT:The recovery of method in different tissues ranged from 96.65% to 105.52%, the RSD of within-day was less than 7.63% and the RSD of between-day less than 11.86%. CONCLUSION:The method is specific, sensitive and rapid, which is suitable for determination of CsA in ocular tissues and for study on its ditribution and pharmacokinetics." @default.
- W2367367032 created "2016-06-24" @default.
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- W2367367032 date "2003-01-01" @default.
- W2367367032 modified "2023-09-25" @default.
- W2367367032 title "Determination of Cyclosporin A in Ocular Tissues by HPLC-MS" @default.
- W2367367032 hasPublicationYear "2003" @default.
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