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- W2367384713 abstract "Objective:To establish polyclonal stable overexpression of prestin(motor protein of outer hair cells in cochlea) in Chinese hamster ovary(CHO) cell line in vitro.Methods:CHO cells were transfected by prestin plasmids,which were selected by high dose(400-800 mg/L) of selective reagent(Zeocin,invitrogen).Polyclonal cells selected by antibiotic(Zeocin) were subcultured and treated by continuous low dose of antibiotic(Zeocin).Selected polyclonal cells were harvested and the total RNA was extracted.Three different primers of prestin protein were designed for RT-PCR.The products of RT-PCR were analyzed with gel electrophoresis.The selected polyclonal cells were analyzed with immunofluorescence staining for expressing of prestin protein.Results:The results of three RT-PCR experiments with different prestin primers were all positive in prestin transfection groups,and negative in control groups.The data of immunofluorescence staining with specific prestin antibody in selected polyclonal cells showed that the positive expression rate of prestin was over 90%.Conclusion:The polyclonal stable overexpression of prestin protein was successfully obtained in CHO cells with high expression rate in transfection cells." @default.
- W2367384713 created "2016-06-24" @default.
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- W2367384713 date "2011-01-01" @default.
- W2367384713 modified "2023-09-23" @default.
- W2367384713 title "Construction of Stable Overexpression of Prestin Protein in CHO Cell Line" @default.
- W2367384713 hasPublicationYear "2011" @default.
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