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- W2367386069 abstract "D-tagatose 3-epimerase(DTE) is the most effective enzyme for the biological production of D-psicose,a novel functional factor.In this study,a novel gene encoding DTE from Clostridium cellulolyticum H10 was cloned.pET-22b(+) was for the plasmid,E.coli BL21(DE3) acted as host cells,then the recombinant strains were constructed.The target protein was over-expressed by IPTG induction;the recombinant DTE purified to electrophoretical homogeneity with affinity chromatography was analyzed by SDS-PAGE,showing approximately 31 000 characteristic protein.The result of activity detection revealed that the recombined enzyme had a higher transforming activity." @default.
- W2367386069 created "2016-06-24" @default.
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- W2367386069 date "2011-01-01" @default.
- W2367386069 modified "2023-09-28" @default.
- W2367386069 title "Cloning,Expression and Characterization of Clostridium cellulolyticum D-Tagatose 3-Epimerase" @default.
- W2367386069 hasPublicationYear "2011" @default.
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