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- W2367500857 abstract "Objective To investigate the role of negative costimulatory signals lymphocyte attenuator(BTLA) and herpes virus entry mediator(HVEM) in murine herpetic stromal keratitis(HSK)mediated by CD4+ T cells by testing the expression of BTLA and HVEM in corneal tissues and CD4+ T cells of peripheral blood.Methods Corneas of BALB/c mice were infected with 106 plague forming unit(PFU) of herpes simplex virus type 1(HSV-1) KOS strain for establishing HSK animal models.About 1 mL orbital venous sinus blood was collected from left eyes of rats before corneal inoculation and on the 3rd,7th,10th,14th,21st day after corneal inoculation with HSV-1.Lymphocytes were segregated and taken fluorescent antibody staining.By flow cytometry,the expression of CD4+ BTLA+ T cells and CD3+ CD4+ HVEM in murine peripheral blood was evaluated.The clinical evaluation of infected eyes was taken under the slit lamp microscope,and the characteristic expression of BTLA and HVEM surface protein on corneas was evaluated by immunohistochemistry.Results Replication of HSV-1 was found in corneal clean and polish liquid at 1 to 5 days after corneal inoculation with HSV-1,which indicated that rats had infected herpes virus.Slit lamp microscope had shown that all rats had infected with acute epithelial keratitis on the 3rd day after corneal inoculation with HSV-1,and cured at 1 week after infection;There were changes of corneal interstitial keratitis on the 8th day after inoculation with HSV-1,showing gray opacity of corneal stroma,went to peak at the 10th day and gradually decreased at the 14th day after corneal inoculation.Flow cytometry detection had shown that positive rates of CD3+ CD4+ BTLA+ T cells and CD3+ CD4+ HVEM+ T cells were(3.15±0.60)% and(9.84±1.06)% at the day after corneal inoculation,reached(20.47±3.15)% and(45.18±3.90)% on the 10th day after corneal inoculation,and at this time the HSK was the most severe.There were statistical differences between the day and on the 10th day after corneal inoculation(both P0.01).Protein expression of BTLA and HVEM were similar in corneal tissues of HSK rats with immunohistochemistry.When the clinical manifestation of HSK was the most severe,at the 10th day after corneal inoculation with HSV-1,BTLA protein and HVSE protein had the highest expression,mainly at inflammatory cells infiltrating corneal stromal layer,and also had expression at corneal epithelium and corneal endothelium.Conclusions In HSK rat models,BTLA and its receptor HVEM have an obviously increased expression in corneal tissues and peripheral blood CD4+ T cells,and costimulatory signal BTLA-HVEM plays a role in immunopathogenesis mechanisms mediated by CD4+ T cells." @default.
- W2367500857 created "2016-06-24" @default.
- W2367500857 creator A5079738340 @default.
- W2367500857 date "2011-01-01" @default.
- W2367500857 modified "2023-09-22" @default.
- W2367500857 title "Aberrant expression of soluble B and T lymphocyte attenuator and its ligand in murine herpetic stromal keratitis" @default.
- W2367500857 hasPublicationYear "2011" @default.
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