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- W2367500979 abstract "Objective:To detect the etiological agent of hand,foot and mouth disease(HFMD)epidemics in Hebei provice from May to August of 2008.Methods:The specimens(including cerebral spinal fluid,vesicle fluid,stool and throat swabs)were inculated into RD cells for isolation of enteroviruses(EV).The isolates were identificated for EV,Enterovirus71(Enterovirus71,EV71) and Coxsackivirus16(CA16) using revers transcription polymerase chain reaction(RT-PCR);50% cell culture infective dose(CCID50) was based cytopathic effect test(CPE).Results:Total 115 viruses were isolated from 203 specimens(56.65%),of which 72 were EV71,42 were EV71+CA16 and 1 was were Non-EV71,CA16 EV.The viruses isolation rate was not satistically significant in different specimens.The virus titration of stools was higher than that of vesicle fluid and throat swabs.Conclusion:EV71 is the main cause of HFMD during the epidemic." @default.
- W2367500979 created "2016-06-24" @default.
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- W2367500979 date "2009-01-01" @default.
- W2367500979 modified "2023-09-23" @default.
- W2367500979 title "Isolation and identification of viruses from patient with hand,foot and mouth disease in Hebei Provice in 2008" @default.
- W2367500979 hasPublicationYear "2009" @default.
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