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- W2367515384 abstract "Objective To research the circadian rhythm of immune cells in the peripheral blood in mice. Methods C57 BL/ 6 male mice were kept in the standardized light-dark cycle( 12 h L: 12 h D) for 4 weeks. Then,peripheral blood samples were collected at 4 time points( 9: 00,15: 00,21: 00 and 3: 00). Flow cytometry was used to detect immune cells,including the total number of white blood cells,the number of lymphocytes,the percentage of CD4+lymphocytes,the percentage of CD8+lymphocytes,the number of monocytes and the percentage of granulocytes. Results In the standardized light-dark cycle,the immune cells in the peripheral blood exhibited a significant circadian rhythm with different apex and nadir. The white blood cells reached apex[( 9. 1 + 1. 8) × 109/ L] at 15: 00 hours,various lymphocytes( CD4+lymphocytes and CD8+lymphocytes) reached apex from 15: 00 to 21: 00 hours. The number of mononuclear cells reached apex( 0. 487 × 109/ L) at 15: 00 hours,while the number of granulocytes reached apex( 0. 127 × 109/ L) at 3: 00 hours. Conclusion In the light-dark cycle,the immune components fluctuated for maintaining the balance of the immune system." @default.
- W2367515384 created "2016-06-24" @default.
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- W2367515384 date "2013-01-01" @default.
- W2367515384 modified "2023-09-24" @default.
- W2367515384 title "Research on the circadian rhythm of immune cells in the peripheral blood in mice" @default.
- W2367515384 hasPublicationYear "2013" @default.
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