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- W2367584012 abstract "[Objective] Preparation of prokaryotic expression construct of Bufo japonicus formosus mcl-1 and the induction of its recombinant protein expression.[Method] The open reading frame(ORF) of B.japonicus formosus skin mcl-1 was amplified by polymerase chain reaction(PCR) and inserted into expression vector pET-28b,and the positive clones were screened by colony PCR and enzyme digestion,which were finally confirmed by DNA sequencing.pET-28b-mcl-1 was transformed into E.coli competent cell BL21(DE3),and the recombinant protein was induced by isopropyl β-D-1-thiogalactopyranoside(IPTG) and its expression was detected by dodecyl sulfate,sodium salt(SDS)-polyacrylamide gel electrophoresis(SDS-PAGE).[Result]The prokaryotic expression construct of pET-28b-mcl-1 was obtained and the expression of the recombinant protein of MCL-1 was confirmed by SDS-PAGE.[Conclusion] This study will become the basis for purifying the recombinant protein and preparation of its antiserum,and the further studies on the biological functions of MCL-1." @default.
- W2367584012 created "2016-06-24" @default.
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- W2367584012 date "2013-01-01" @default.
- W2367584012 modified "2023-09-25" @default.
- W2367584012 title "Preparation of Prokaryotic Expression Construct of Bufo japonicus formosus MCL-1 and Its Recombinant Protein Expression" @default.
- W2367584012 hasPublicationYear "2013" @default.
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