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- W2367600708 abstract "The binary vectors pCAM3300 was introduced into Agrobacterium tumefaciens by electroporation. Effects of different electroporation conditions on transformation frequency were studied. The results suggested transformation frequency reached to 9.8×10~(3) CFU/μg DNA, the highest level, when the electrical field strength was 11 kV/cm. Transformation frequency also reached a highest level of 9.54×10~(3)CFU/μg DNA when OD_(600) was at about 1.0. The transformation frequency was related to cell density, it increased with the increase of cell concentration. However, the size of plasmid had no specific relation with electroporation. The A. tumefaciens containing the binary vectors pCAM3300 co-cultured with Neurspora crassa and transformants were gained on plate containing 405 mg/L of PPT. PCR results demonstrated that Bar gene was integrated into chromosome of Neurspora crassa." @default.
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- W2367600708 date "2005-01-01" @default.
- W2367600708 modified "2023-09-24" @default.
- W2367600708 title "Studies on Electroporation Transformation of Agrobacterium tumefaciens" @default.
- W2367600708 hasPublicationYear "2005" @default.
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