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- W2367605549 abstract "According to characteristics of the foot and mouth disease virus infection in the pathogenesis of the clinical presentation of symptoms,screening single-chain antibody against foot and mouth disease virus gene.By EcoRⅠ/NotⅠ double digestion to obtain intermediate plasmid pGEX-ScFv single chain antibody gene fragment and cloned into retroviral vector plasmid pFB-neo,to build anti-retroviral shuttle vector plasmid pFB-ScFv.PFB-ScFv with the helper plasmid pVPack-GP,pVPack-10A1 were cotransfected into HEK293T cells,packaging recombinant retroviruses MMLV-ScFv.Meanwhile,building enhanced green fluorescent protein recombinant retroviruses MMLV-EGFP as a control.Useing recombinant retrovirus infect swine fibroblasts,obtained the target gene carrying monoclonal colony by G418 selection.Restriction endonuclease detection,fluorescence detection and reverse transcription polymerase chain reaction and other experimental results show that,In this study,based on the successful construction of anti-retroviral shuttle vector pFB-ScFv and pFB-EGFP,obtained anti-FMD virus were carrying single-chain antibody gene and enhanced green fluorescent protein retrovirus.And eventually were selected to carry the gene cloned pig fibroblasts for the anti-FMD virus transgenic animal research laid the foundation." @default.
- W2367605549 created "2016-06-24" @default.
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- W2367605549 date "2012-01-01" @default.
- W2367605549 modified "2023-09-26" @default.
- W2367605549 title "Establishment of the anti-FMDV transgenic pig fibroblasts cell lines" @default.
- W2367605549 hasPublicationYear "2012" @default.
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