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- W2367609268 abstract "Porcine interleukin-2 and porcine interleukin-6 cDNA sequences were cloned into the expressing plasmid vectors pET-28a and pGEX-KG, respectively; and expressed in E. coli BL21(DE3) with high-level production. The gene deleted vaccine of pseudorabies virus Ea strain ( TK- /gG- /LacZ + ) as mixed with the two different purified recom-binant proteins each, or both, with the doses of 2, 5 and 10ug.ml-1. Ten groups of pseudorabies negative antibody swines were immunized twice with tested vaccines at different doses, or control vaccine, respectively. The antibody titers of the test groups were delected by neulralizalion test, and the daily weight gains of swines were calculated and analyzed statistically. It was found that all the neutralizing anlibody titers in lest groups were higher than the control group, and the recombinant proleins appeared a dose dependenl adjuvant effect. A significant and extremely significant differences, were observed in tested vaccines containing 2 ug.ml-1 pIL-2 and with 10ug.ml-1 pIL-2/pIL-6, and the vaccines with-out pILs. The difference of the daily weighl gain indicated the potenlial posilive influence of pIL-2 and pIL-6 on immune proteclion." @default.
- W2367609268 created "2016-06-24" @default.
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- W2367609268 date "2003-01-01" @default.
- W2367609268 modified "2023-09-24" @default.
- W2367609268 title "Expression of Porcine linterleukin-2 and Porcine lnterleukin-6 and their Adjuvant Effects on Gene Deleted Vaccine of Pseudorabies Virus (TK-/gG-/LacZ+)" @default.
- W2367609268 hasPublicationYear "2003" @default.
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