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- W2367633241 abstract "To product monoclonal antibody against Hsp16.3 of Mycobacterium tuberculosis,and identify its character.The recombinant pProEXHTb-Hsp16.3 plasmid was transferred into E.coli DH5α,and induced by IPTG.The Hsp16.3 protein with 6×His was purified by Ni-NTA purification system under denaturing condition.Following by renaturation of dialysis,Hsp16.3 was used as antigen to immunize the BALB/c mice and product monoclonal antibodies.The positive clone was identified through indirect ELISA.The mAb specificity was assayed by ELISA and Western blot.Three anti-Hsp16.3 mAbs,named 3H6F9,1D5E1,4H8G6 respectively,were obtained.The titer and relative affinity of the three mAbs were 1:1×107,1:1×106,1:1×106 and 0.000 1 mg/mL,0.001 mg/mL,0.001 mg/mL.It could specially bind to Hsp16.3.The anti-Hsp16.3 mAbs with high specificity and high affinity were successfuly obtained,which were the effective tools to research the role of Hsp16.3 in Mycobacterium tuberculosis latent infection." @default.
- W2367633241 created "2016-06-24" @default.
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- W2367633241 date "2012-01-01" @default.
- W2367633241 modified "2023-09-26" @default.
- W2367633241 title "Production and Identification of Monoclonal Antibodies Against Mycobacterium tuberculosis Heat Shock Protein 16.3" @default.
- W2367633241 hasPublicationYear "2012" @default.
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