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- W2367688107 abstract "Objective To develop a quantitative analysis method for determination of metoprolol enantiomer by HPLC.Methods Metoprolol was separated by HPLC on a Chiralcel AD-H column(250 mm×4.6 mm,5 μm) with a mixture of n-hexane-alcohol-diethylamine as mobile phase.Results The mobile phase was N-hexane-alcohol-diethylamine(90:10:0.1),the flow rate was 1.0 ml/min,and the column temperature was 30 ℃.The calibration curve of metoprolol was linear in the range of 0.1 to 0.9 mg/ml,and the relative standard deviation was 0.46%(n= 8).Metoprolol had a good relationship:(R)-metoprolol: Y = 8.9×106X + 1.0×106,r=0.999 5;(S)-metoprolol: Y=9.8×106X + 8.1×105,r=0.999 3,R=3.64,separation factor was 1.85.Conclusion This method is sensitive,reproducible,accurate and reliable." @default.
- W2367688107 created "2016-06-24" @default.
- W2367688107 creator A5062572385 @default.
- W2367688107 date "2011-01-01" @default.
- W2367688107 modified "2023-09-23" @default.
- W2367688107 title "Analysis of Metoprolol enantiomer by High Performance Liquid Chromatography" @default.
- W2367688107 hasPublicationYear "2011" @default.
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