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- W2367710466 abstract "AIM: To study whether there is significant difference in the inductive effects on CYP3A4 between human vitamin D Receptor(hVDR) wild type and start codon Fok Ⅰ mutant.METHODS: Total RNA was extracted from liver samples.cDNA of hVDR wild type was acquired by RT-PCR,and cDNA with hVDR Fok Ⅰ mutant was acquired using site-directed mutagenesis.Eukaryotic expression vectors of both wild type and mutant of hVDR were constructed according to cDNA.Meanwhile,luciferase reporter gene vector was constructed with proximal ER6 element of CYP3A4.COS-7 cells with hVDR eukaryotic expression vectors,CYP3A4 luciferase reporter gene vector and the internal control vector were transiently transfected for 24 h and then were incubated with 1 nmol/L,10 nmol/L and 100 nmol/L 1,25(OH)2D3 for 48 h.Finally the cells were lysed and the luciferase activity was detected with cell lysis.RESULTS: hVDR eukaryotic expression vectors of wild type and Fok Ⅰ mutant,and CYP3A4 luciferase reporter gene vector were successfully constructed.The results of luciferase activity showed that CYP3A4 transcription could be activated by hVDR eukaryotic expression vectors of wild type and Fok Ⅰ mutant when incubated with 1 nmol/L,10 nmol/L and 100 nmol/L 1,25(OH)2D3,respectively.However,there was no significant difference between the luciferease activities of hVDR wild type and mutant.CONCLUSION: Both hVDR wild type and Fok Ⅰ mutant have inductive effect on CYP3A4 when incubated with vitamin D3(VD3).But Fok Ⅰ mutant could not significantly change the regulational capacity of wild type VDR on CYP3A4." @default.
- W2367710466 created "2016-06-24" @default.
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- W2367710466 date "2009-01-01" @default.
- W2367710466 modified "2023-09-23" @default.
- W2367710466 title "Effects of vitamin D receptor Fok I polymorphism on the transcriptional control of CYP3A4" @default.
- W2367710466 hasPublicationYear "2009" @default.
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