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- W2367714653 abstract "The expression of two important regulatory factors(microRNA156b(Vv-miR156b)and microRNA172c(Vv-miR172c)),their target genes and their interaction during the second flower development in winter buds growing on shoots of grapevine'Fujimori'subjected to cut-back treatments at various times were studied.Semi-quantitative and quantitative real-time RT-PCR were employed in analyzing variations in the expression levels of the two miRNAs,while RLM-5′-RACE was used to validate the modes by which these miRNAs regulate their target genes.Observations showed that grapevine winters only in the third topping treatment could have flower bud differentiation and form inflorescences while no flower bud differentiation occurred in the other treatments and control winters.Two RT-PCR results with identity showed that both miRNAs exhibited distinctly decreased expression and were least expressed in the inflorescence,while the corresponding target genes for these miRNAs exhibited inverse expression trends with those of their miRNAs.The RLM-5′-RACE results showed that Vv-miR156b and Vv-miR172c could negatively regulate expression of their target genes by cleavage,and their target cleavage sites were between the tenth and eleventh nucleotide at the 5′-end of the miRNA.All results suggested that microRNA156b and microRNA172c were involved in the process of the second flower development of grape winters through regulating expression levels of target genes by cleavage." @default.
- W2367714653 created "2016-06-24" @default.
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- W2367714653 date "2012-01-01" @default.
- W2367714653 modified "2023-09-23" @default.
- W2367714653 title "Spatiotemporal expression of microRNA156b and microRNA172c and their target genes during flower development of winter buds growing on cut-back treated shoots of grapevine" @default.
- W2367714653 hasPublicationYear "2012" @default.
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