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- W2367716100 abstract "Objective To use a quantum dots labeled probes fluorescence in situ hybridization(FISH) method for rapid identification of Staphylococcus aureus.Methods Two quantum dots-avidin-biotin-DNA probes,type A enterotoxin(SEA) gene probe and FemB gene probe,were used to perform FISH with clinically isolated Staphylococcus aureus or fecal sample of Staphylococcus aureus infected patients.The results were compared with that of polymerase chain reaction(PCR) of SEA gene.Results Of the 10 clinically isolated Staphylococcus aureus strains,2 were positive and 8 were negative for both the SEA gene probe hybridization and PCR tests,while all 10 were positive for FemB gene probe hybrizidation test.Three fecal samples had Staphylococcus aureus isolated,and all these 3 fecal samples were positive for both the SEA and FemB gene probe hybridization test.SEA gene PCR test was all positive also.No non-specific reaction was seen between SEA,FemB gene probes and that of other related bacterial species.Conclusions Quantum dots labeled probes fluorescence in situ hybridization is a simple and rapid method for identifying Staphylococcus aureus,even in stool samples." @default.
- W2367716100 created "2016-06-24" @default.
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- W2367716100 date "2010-01-01" @default.
- W2367716100 modified "2023-09-25" @default.
- W2367716100 title "Quantum dots labeled probes fluorescence in situ hybridization for rapid detection of Staphylococcus aureus" @default.
- W2367716100 hasPublicationYear "2010" @default.
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