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- W2367716610 abstract "According to the coding sequences of Bos taurusinterferon induced transmembrane proteins(bIFITMs),the primers were designed and synthesized.Then the coding sequences were obtained by RT-PCR and subcloned to pMD18-T simple vectors.After sequencing,homologous alignments and key sites analysis of IFITMs sequence were performed through bioinformatics software DNAStar,DNA MAN and MEGA 6.0.The phylogenetic trees of target genes were also constructed.Meanwhile,the target genes were inserted respectively into the eukaryotic expression vector pLV-EGFP,resulting into pLV-bIFITM1,pLV-bIFITM2,pLV-bIFITM3.Then these recombinant plasmids were transfected into HEK293 T,BHK-21.The expression of target genes were detected by RT-PCR,Western blot.The results showed that the coding sequences of bIFITM1,bIFITM2,bIFITM3 was obtained successfully and the relevant biological informations was built.The pLV-bIFITM1,pLV-bIFITM2 and pLV-bIFITM3 eukaryotic expression plasmids were constructed successfully and IFITM proteins can be effectively expressed in eukaryotic cells,providing experimental foundations for further exploring the antiviral actions of bovine IFITMs and animal transgenic antiviral breeding." @default.
- W2367716610 created "2016-06-24" @default.
- W2367716610 creator A5011626892 @default.
- W2367716610 date "2015-01-01" @default.
- W2367716610 modified "2023-09-24" @default.
- W2367716610 title "Cloning,sequence analysis and expression of Bos taurus interferon induced transmembrane protein genes" @default.
- W2367716610 hasPublicationYear "2015" @default.
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