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- W2367738225 abstract "Objective To synthesize the gene encoding human GnRH and its transporter(TRS).Methods Design a pair of nucleotide sequences according to the reported gene encoding human GnRH and its transporter and purify by PAGE with denaturing polyacrylamide gel containing 7 mol/L urea.Synthesize a 90 bp target sequence GnRH/TRS by filling-in of a short complementary sequence at 3′-terminus of this pair of nucleotide sequences.Subclone the synthetic sequence to pMD18-T vector to construct recombinant plasmid pYC1.Identify the positive clones by restriction analysis and sequencing.Results The synthetic GnRH/TRS sequence consisted 90 nucleotides and showed a homology of 100% to that designed.Conclusion Human GnRH/TRS gene was successfully synthesized.It laid a foundation of further study and application of GnRH/TRS." @default.
- W2367738225 created "2016-06-24" @default.
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- W2367738225 date "2006-01-01" @default.
- W2367738225 modified "2023-09-25" @default.
- W2367738225 title "Synthesis and Identification of Gene Encoding Human GnRH and Its Transporter" @default.
- W2367738225 hasPublicationYear "2006" @default.
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