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- W2367748960 abstract "In order to determine the optimum conditions for extraction and isolation of 11S and 7S fractions from soybean storage protein,the factors affecting the extraction and isolation(the kinds of extraction solution,alkaline extraction pH,times of extraction and temperature,meal-to-water ratio,Tris-HCl concentration,the kinds of reducing agent)were investigated by Kjeldahl method and SDS-PAGE,and further more Nagano method was modified.The results indicated that the defatted soybean meal was extracted with 0.03～0.06 mol/L Tris-HCl buffer(pH 8.5)containing 0.01 mol/L sodium bisulfite(SBS)at 45℃ for 1 hour.The extraction was repeated twice with meal-to-water ratio(1∶15).The 11S fraction was precipitated at pH 6.4 and isolated by centrifugation,and then the pH of the supernatant was adjusted to pH 5.5 to remove the insoluble intermediate fraction by centrifugation.After then,the supernatant was adjusted to pH 4.8 to precipitate and isolate the 7S fraction by centrifugation.Under these conditions,the yield,protein content and purity of 11S and 7S fractions could be increased significantly than that by Nagano method." @default.
- W2367748960 created "2016-06-24" @default.
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- W2367748960 date "2006-01-01" @default.
- W2367748960 modified "2023-09-24" @default.
- W2367748960 title "Optimization of extraction and isolation of 11S and 7S fractions from soybean storage protein" @default.
- W2367748960 hasPublicationYear "2006" @default.
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