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- W2367760416 abstract "Objective To study the influence of norcantharidin (NCTD) on proliferation and apoptosis of GBC SD cell lines of human gallbladder carcinoma in vitro. Methods GBC SD cell lines of human gallbladder carcinoma were cultured by cell culture technique. The tetrazolium based colorimetric assay was used to evaluate the growth inhibitory effect of NCTD. Flow cytometry was used to measure the induction of cell cycle arrest and apoptosis. Microscope and scanning electron microscope were used to observe the morphological changes of the cells. Results NCTD displayed the inhibitory effect on growth of GBC SD cells from the concentration of 10 μg/ml. It was in a dose and time dependent manner with the IC 50 value of 60 μg/ml for 72 h. The flow cytometric profiles revealed that NCTD led to a shift from 28.54% up to 58.71% in the percentage of GBC SD cells in G2/M phase and a shift from 24.47% down to 6.33% of the S phase cells, and increased the rate of cell apoptosis. After treatment with 60 μg/ml NCTD for 48 h, cell shrinkage, membrane budding, and karyorrhexis of some GBC SD cells could be seen by microscope; decrease of microvillis, atrophy of Golgi apparatus and mitochondria and typical apoptotic cells could also been seen by electron microscope. Conclusion NCTD can inhibit the growth of GBC SD cells of human gallbladder carcinoma. The mechanism might be related to the induction of cell apoptosis, inhibition of cell proliferation, blockade of cell cycle, and inhibition on DNA synthesis, influence of cell metabolism." @default.
- W2367760416 created "2016-06-24" @default.
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- W2367760416 date "2003-01-01" @default.
- W2367760416 modified "2023-09-28" @default.
- W2367760416 title "Influence of norcantharidin on proliferative growth and apoptosis of GBC-SD cell lines of in human gallbladder carcinomas." @default.
- W2367760416 hasPublicationYear "2003" @default.
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