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- W2367766047 abstract "Combined nucleotide sequence of four gene fragments of mitochondrial DNA(mtDNA),which were control region,cytochrome b gene,16S rRNA gene and COⅡ gene,was analyzed for five populations of blunt snout bream(Megalobrama amblycephala) with different ploidy levels,which were Auto-4n-F1,positive 3n hybrid,negative 3n hybrid,allo-3n and 2n,to detect their genetic variations.The lengths of mitochondrial DNA control region,cytochrome b gene,16S rRNA gene and COⅡ gene were 937 bp,1 140 bp,564 bp and 642 bp, respectively. These four gene fragments were combined to form a 3 283 bp long gene fragment. The average contents of nucleotides T,C,A,G were 27.18%,25.45%,30.71% and 16.66%, respectively in the 3 283 bp long sequence. 55 haplotypes were defined in the 57 individual,without haplotype shared among populations. The diversity of haplotypes were 0.984 8~1.000 0,indicating the abundance of haplotypes within different populations. The nucleotide differences and the nucleotide diversity within each population were 8.000~24.273, and 0.248 3 %~0.786 6%,respectively. Genetic distance between populations were 0.003 9~0.010 6. The above results indicated that genetic diversity in five populations of blunt snout bream with different ploidy levels was very high. There was the same trend in the change of nucleotide diversity (π),average number of nucleotide differences(K) and average genetic distances of nucleotide sequences among five populations,that is to say, frow high to low is:negative 3n hybrid,allo-3n,positive 3n hybrid,auto-4n-F1,2n. What’s more,nucleotide diversity (π),average number of nucleotide differences(K) and average genetic distances of nucleotide sequences of negative 3n hybrid,allo-3n,positive 3n hybrid and auto-4n-F1 were significantly higher than those of 2n(P0.05). It could be concluded that the genetic diversity within negative 3n hybrid,allo-3n,positive 3n hybrid and auto-4n-F1 populations were significantly higher than that of 2n population." @default.
- W2367766047 created "2016-06-24" @default.
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- W2367766047 date "2008-01-01" @default.
- W2367766047 modified "2023-09-23" @default.
- W2367766047 title "Analysis of mitochondrial DNA in blunt snout bream(Megalobrama amblycephala) populations with different ploidy levels" @default.
- W2367766047 hasPublicationYear "2008" @default.
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