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- W2367780586 abstract "Objective To construct the recombinant eukaryotic expression vector of HMGA2 gene and observe its expression in eukaryotic cells,and laid a theoretical foundation for further study of HMGA2 gene function.Methods HMGA2 gene full-length cDNA from human breast epithelial cell HBL-100 was amplified by RT-PCR;after double-digestion and gal recovery HMGA2 gene was cloned into YFP-C1 eukaryotic expression vector.After eukaryotic expression vector YFP-C1-HMGA2 was transfected into human prostate cancer cells,and the correctness of constructed YFP-C1-HMGA2 was certificated by RT-PCR.Results HMGA2 gene full-length cDNA of 351 bp was obtained by RT-PCR.After the cloning of YFP-C1 eukaryotic expression vector,double enzyme digestion and sequencing analysis,YFP-C1-HMGA2 eukaryotic expression vector was confirmed to be successful.And it could express in eukaryotic cells.Conclusion HMGA2 gene is cloned successfully,and YFP-C1-HMGA2 eukaryotic expression vector is constructed successfully." @default.
- W2367780586 created "2016-06-24" @default.
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- W2367780586 date "2009-01-01" @default.
- W2367780586 modified "2023-09-23" @default.
- W2367780586 title "Construction and expression identification of eukaryotic expression vector of HMGA2 gene" @default.
- W2367780586 hasPublicationYear "2009" @default.
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