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- W2367846886 abstract "R-2’-chloro-1-phenyl-ethanol was gained by asymmetric reduction of 2’-chloroacetophenone with Saccharomyces cerevisiae B5 in the presence of ethanol(5%v/v) as co-substrate. A kinetic model descripting substrate consumption and product accumulation coupled with coenzymes regeneration process was proposed. The type and the content of coenzyme, and the changes of the substrate and product content vs. time during the reaction process were investigated. The results indicate that coenzymeⅠis reducer. The poison of organic substrate on microorganism can be ignored when substrate initial concentration is lower than 8.09 mmol·L?1. The whole reaction is a combination of two oxidation-reduction reactions which conform with sequence mechanisms. The model parameters obtained from the fitting of experimental data are:Vm1 = 8.0×10?4 mol?L?1?h?1,KmB1 = 9.0 × 10?4 mol?L?1,KiA1 = 2.0 × 10?6 mol?L?1. The results of the model agree well with the experimental data." @default.
- W2367846886 created "2016-06-24" @default.
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- W2367846886 date "2005-01-01" @default.
- W2367846886 modified "2023-09-28" @default.
- W2367846886 title "Kinetic Model of Asymmetric Reduction of 2’-chloroacetophenone by Saccharomyces cerevisiae B5 with Coenzyme Regeneration Process" @default.
- W2367846886 hasPublicationYear "2005" @default.
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