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- W2367939377 abstract "Objective In order to obtain the recombinant proein EgmMDH( E. granulosus mitochondrial malate dehydrogenase),the recombinant His6-tagged EgmMDH from the extract of transformed E. coli BL21 was expressed and refolded. Method Firstly,the mdh gene was isolated from Echinococcus granulosus and cloned as described by our lab before. Plasmid pET28a-mMDH was transformed into E. coli BL21. rEgmMDH was expressed in plasmids of pET28a-mMDH / BL21. Secondly,the recombinant His6-tagged EgmMDH was purified by nickel chelate affinity chromatography( Novagen). The purified His6-tagged protein was refolded by dialysis method. Then,The rabbits were challenged infection with Echinococcus granulosus( Chinese mainland strain) to obtain anti-rabbit sera. At last,the renaturalized rEgmMDH was detected by western blot using anti-rabbit sera and anti-his-tag monoclonal antibody. Results ① pET28a-mMDH / BL21 plasmid was constructed successfully. ② The high purified soluble protein was harvested after the His6-tagged rEgmMDH protein was purified and refolded. ③ The renaturalized rEgmMDH could be recognized with specific antibodies in western blotting. Conclusion In this study,a purified soluble rEgmMDH was expressed and identified successfully,which will be as a basis for further study on hosts and E. granulosus interplay." @default.
- W2367939377 created "2016-06-24" @default.
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- W2367939377 date "2014-01-01" @default.
- W2367939377 modified "2023-09-28" @default.
- W2367939377 title "Expression,Renaturation and Identification of Recombinant mMDH of Echinococcus Granulosus( Chinese mainland strain)" @default.
- W2367939377 hasPublicationYear "2014" @default.
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