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- W2367957264 abstract "AIM:To clone and analyze the encoding region of heme oxygenase-1(HO-1) gene from Tibetan antelope(Pantholops hodgsonii).METHODS: The total RNA was isolated from the liver of Tibetan antelope and the HO-1 gene was amplified by RT-PCR.The PCR product was cloned into pGEM-T vector and sequenced.The nucleotide sequences were compared with the data from GenBank by BLAST method.RESULTS: The encoding region of HO-1 gene from Tibetan antelope was obtained and deposited in GenBank as accession number JQ809687.The encoding sequence was 897 bp in full length,which encoded 298 amino acids.Sequence similarity analysis showed that the HO-1 gene cloned from Tibetan antelope shared 98% and 96% in the nucleotides,and shared 92% and 97% in the amino acids with those of Capra hircus and Bos taurus,respectively.The sequence similarity of nucleotides and amino acids also shared 86% and 87% with other vertebrates,which were highly conservative.The molecular phylogenetic tree based on the amino sequence of HO-1 showed that Tibetan antelope and Capra hircus was classified to one cluster,which was basically concerted to the evolutionary relationship of traditional species.CONCLUSION: The encoding region of Tibetan antelope HO-1 gene is successfully cloned,which provides a foundation for exploring the molecular and biological mechanisms of high altitude adaptation and cell protection in Qinghai-Tibet Plateau species under hypoxic condition." @default.
- W2367957264 created "2016-06-24" @default.
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- W2367957264 date "2012-01-01" @default.
- W2367957264 modified "2023-09-25" @default.
- W2367957264 title "Cloning and sequencing of encoding region of heme oxygenase-1 gene from Tibetan antelope" @default.
- W2367957264 hasPublicationYear "2012" @default.
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