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- W2367967440 abstract "Objective To establish a real time RTPCR method for detecting the expression of BDNF mRNA(brain-derived neurotrophic factor messenger ribonucleic acid,BDNF mRNA) in cerebrum tissue after MCAO/R(middle cerebral artery occlusion reperfusion,MCAO/R) injury in rats.Methods Total RNA was isolated from cerebrum tissue.Specific oligonucleotide of BDNF mRNA gene fragments were amplified by RT-PCR.The products of RT-PCR were recombined to plasmid and sequenced.The method of detecting BDNF mRNA expression by real time RT-PCR was established.Results The recombinant plasmid was digested by enzymes and sequenced.It was proved that the specific fragment had been cloned to vectors.The kinetics curves reflecting the progress of gene amplification and a quantitative standard curve were completed.Conclusions The method of detecting BDNF mRNA expression by real time RT-PCR is constructed successfully." @default.
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- W2367967440 date "2007-01-01" @default.
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- W2367967440 title "Establishment of A Real Time Method for Detecting the Expression of BDNF mRNA Gene" @default.
- W2367967440 hasPublicationYear "2007" @default.
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